Self-contained biological indicators (SCBI’s) are available for monitoring the efficacy of Steam, H2O2/ Plasma and Ethylene Oxide/ Dry heat sterilization processes.
SCBI’s contain bacterial spores on a paper carrier (or tyvek disc for H2O2/plasma) plus a sealed glass ampoule within a vented plastic vial.
The glass ampoule contains a proprietary formulation of soybean casein digest culture media with a pH indicator. The pH indicator remains purple when there is no spore growth and yellow when there is spore growth. This allows quick visual determination of sterilization failures (yellow colour).



Sterilization Process

 Incubation time

 Incubation temperature

 Etigam Productcode

Geobacillus stearothermophilus 10^5 Steam 24 hours 60 C NSC-S5
Geobacillus stearothermophilus 10^6 Steam 24 hours 60 C NSC-S6
Geobacillus stearothermophilus 10^5 Steam 10 hours 60 C NRI-S5
Geobacillus stearothermophilus 10^6 Steam 10 hours 60 C NRI-S6
Geobacillus stearothermophilus 10^6 H202 / Plasma 24 hours 60 C NSC-H6
Bacillus Atrophaeus (Subtilis var. Niger) 10^6 EO gas / Dry heat 48 hours 37 C NSC-E6