Biological indicators (BI’s) are generally accepted as the most suitable device to use for monitoring sterilization processes on their effectiveness and the lethality of the used sterilizer. The BI’s contain a specific micro organism that is proven to be highly and stable resistant to the used sterilization process. The BI’s are used to assist in sterilization processes that render a product sterile in its final package or container, as well as for the sterilization of equipment, materials, and packaging components used in aseptic processing. Etigam BI’s are conventional spore growth readout indicators specifically designed for reliable monitoring of sterilization processes without the use of enzyme based technology or specific and specialized incubators or monitoring devices. Etigam NSS BI’s comply with the performance requirements of ANSI/AAMI/ISO 11138-1 and the USP requirements.